Insulin Like Growth Factor Binding Protein -1 ( Igfbp-1)

The iGFBP-1 (vaginal secretion) has been designed to detect iGFBP-1 through visual
interpretation of color development in the internal strip. The membrane was immobilized with antiiGFBP-1 antibodies on the test region. During the test, the specimen is allowed to react with
colored anti-iGFBP-1 antibodies colloidal gold conjugates, which were precoated on the sample
pad of the test. The mixture then moves on the membrane by a capillary action, and interacts
with reagents on the membrane. If there was enough iGFBP-1 in specimens, a colored band will
form in the T region of the membrane. Presence of colored band indicates a positive result, while
its absence indicates a negative result. Appearance of a colored band in the control region
serves as a procedural control. This indicates that proper volume of specimen has been added
and membrane wicking has occurred